Western blot (WB) of total cell extracts from (a

d) human Jurkat, (c) human HepG2; using 2 independent Abs against 2 distinct regions of mouse MLXIPL at RT for 2 h. |IP-WB. Immunoprecipitaion (IP) was performed with HeLa or Jurkat extracts with IgG or anti-MLXIPL (C) (Orb67303) as indicated, followed by WB with anti-MLXIPL (N2) (Orb67302). |Total extracts from human HepG2 were immunoprecipitated with IgG or anti-MLXIPL (C) (Orb67303) under the conventional IP conditions or cross-linked chromatin immunoprecipitation (ChIP) conditions; followed by WB with anti-MLXIPL (N2) (Orb67302) at RT.